Research Supports Double Defense Heartworm Protocol


It’s time to fight both parasites involved in the heartworm lifecycle—and that means fighting the vector as well as the disease.

It’s time to hold the mosquito accountable for the transmission of heartworm disease.

It’s time for Double Defense.

Double Defense Heartworm Protocol is an approach that protects dogs from mosquitoes and heartworms.1,2 This standard of care means that every dog should be on a macrocyclic lactone year-round and a mosquito repellent.

Groundbreaking studies by a third-party investigator, Dr. John McCall, MS, PhD, support Double Defense Heartworm Protocol in the fight against heartworms.


Repelling and killing mosquitoes is effective in prohibiting microfilariae transmission from dogs to mosquitoes.2


Dogs protected with Double Defense had no adult heartworms even using the JYD-34 strain of Dirofilaria immitis (one of the most resistant strains known).1

  • In the studies using the Double Defense approach, there were:
  • Fewer mosquito bites2
  • Fewer infected mosquitoes2
  • Fewer infected dogs2
  • Fewer L3 transmitted1
  • No adult heartworms in dogs1

This vector-targeted approach has been proven effective in addressing different mosquito-borne diseases in humans. Effective use of mosquito repellents is a basic tool in the fight against malaria, West Nile and Zika. Now, Dr. McCall’s research proves that it is time to bring the same approach to preventing canine heartworm transmission.

Do more to protect dogs from deadly heartworms.

Learn more about Double Defense at and hear from other experts who have weighed in on the studies.


1. McCall, J.W., Hodgkins, E., Varloud, M., Mansour, A., DiCosty, U., McCall, S., Carmichael, J., Carson, B., Carter, J. (2016, August). Blocking of the transmission of Dirofilaria immitis L3 (JYD-34 ML resistant strain) from infected mosquitoes to dogs and prevention of infection in dogs treated topically with dinotefuran-permethrin-pyriproxyfen and orally with milbemycin oxime alone or in combination. Abstract presented at the meeting of the American Association of Veterinary Parasitologists, San Antonio, TX.

2. McCall, J.W., Hodgkins, E., Varloud, M., Mansour, A., DiCosty, U. (2015, July). Inhibition of the transmission of Dirofilaria immitis to mosquitoes by weekly exposure to microfilaremic dogs treated topically with dinotefuran–permethrin-pyriproxyfen. Abstract presented at the meeting of the American Association of Veterinary Parasitologists, Boston, MA

3. Capelli et al., Risk of canine and human exposure to Dirofilaria immitis infected in endemic areas of Italy. Parasites & Vectors 2013, 6:60. 

Photo credit: (c) iStock/doug4537

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